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Gibbs' Rules:

Rule #1: Never let suspects stay together. (Franks) 1x01 'Yankee White'

Rule #1: Never screw over your partner. 4x14 'Blowback' 

Rule #2: Always wear gloves at a crime scene.
(Franks) 1x01 'Yankee White'

Rule #3: Don't believe what you're told. Double check.
(Franks) 1x01 'Yankee White'

Rule #3: Never be unreachable. 3x13 'Deception'

Rule #4: The best way to keep a secret? Keep it to yourself.
Second best? Tell one other person - if you must.
There is no third best. 4x11 'Blowback'

Rule #6: Never apologize — Its a sign of weakness. A rule used more often than the others, but finally given a number in 7x12 'Flesh and Blood' during a great Gibbs/DiNozzo moment. The first person to use that phrase was John Wayne in, "She Wore a Yellow Ribbon." "Never apologize, mister, it. It's a sign of weakness." — In the episode 3x23 'Hiatus Part-1' DiNozzo explains to NCIS Director, Jenny Shepard, that Gibbs took this one from "The Duke himself."

Rule #7: Always be specific when you lie.
1x23 'Reveille'

Rule #8: Never take anything for granted.
3x10 'Probie'

Rule #9: Never go anywhere without a knife.
1x13 'One Shot, One Kill' & 1x20 'Missing'

Rule #10: Never get personally involved on a case. (Said by Tony, agreed on by Gibbs)
7x21 'Obsession'

Rule #11: When the job is done, walk away.
6x24 'Semper Fidelis'

Rule #12: Never date a coworker. 1x15 'Enigma'

Rule #13: Never, ever involve a lawyer. 6x07 'Collateral Damage' & 7x24 'Rule Fifty-One'

Rule #15: Always work as a team. 5x05 'Leap of Faith'

Rule #18: It's better to seek forgiveness than ask permission. 3x04 'Silver War'

Rule #22: Never, ever bother Gibbs in interrogation.
4x10 'Smoked'

Rule #23: Never mess with a Marine's coffee if you want to live.
2x09 'Forced Entry'

Rule #27: Two ways to follow: — First way they never notice you, — second way they only notice you. 7x15 'Jack Knife' &
7x24 'Rule Fifty-One'

Rule #38: Your case, your lead. 6x16 'Bounce'

Rule #39: There is no such thing as coincidence.
7x21 'Obsession'

"The 40's are for Emergency Use Only!"
7x24 'Rule Fifty-One' (Mentioned by Abby)

Rule #40: If it seems someone is out to get you, they are.
(Said by Abby, agreed on by Gibbs) 7x22 'Borderland'
& 7x24 'Rule Fifty-One'

Rule #44: First things first. Hide the women and children. 7x23 'Patriot Down'

Rule #45: *PENDING* Gibbs Said, "Left a mess I've got to clean up." 7x24 'Rule Fifty-One' (See Below)

Rule #51: Sometimes — You're Wrong! 7x24 'Rule Fifty-One'